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Center for Data and Simulation Science

As a member of the CCG’s bioinformatics team, my work focuses on the continuous operation and maintenance of the CCG’s NGS analysis pipelines for monogenic disease research. Here, the optimization of the workflows for robust and efficient operation in the HPC environment provided by the Regional Computing Center of the University of Cologne (RRZK) is the main goal. Additionally, I do custom analyses of smallRNA sequencing experiments and CNV analyses in exome or genepanel cohorts. Analysis results can be comfortably accessed and reviewed through the CCG’s Varbank database and webinterface continuously advanced by the CCG’s bioinformatics team (https://varbank.ccg.uni-koeln.de).

The CCG’s exome analysis pipeline.

Selected publications

  1. Awazawa M, Gabel P, Tsaousidou E, Nolte H, Krüger M, Schmitz J, Ackermann PJ, Brandt C, Altmüller J, Motameny S, Wunderlich FT, Kornfeld JW, Blüher M, Brüning JC. A microRNA screen reveals that elevated hepatic ectodysplasin A expression contributes to obesity-induced insulin resistance in skeletal muscle. Nat Med. 2017 Dec;23(12):1466-1473. doi: 10.1038/nm.4420. Epub 2017 Nov 6.
  2. van Doormaal PTC, Ticozzi N, Weishaupt JH, Kenna K, Diekstra FP, Verde F, Andersen PM, Dekker AM, Tiloca C, Marroquin N, Overste DJ, Pensato V, Nürnberg P, Pulit SL, Schellevis RD, Calini D, Altmüller J, Francioli LC, Muller B, Castellotti B, Motameny S, Ratti A, Wolf J, Gellera C, Ludolph AC, van den Berg LH, Kubisch C, Landers JE, Veldink JH, Silani V, Volk AE. The role of de novo mutations in the development of amyotrophic lateral sclerosis. Hum Mutat. 2017 Nov;38(11):1534-1541. doi: 10.1002/humu.23295. Epub 2017 Aug 3.
  3. Altmüller J, Motameny S, Becker C, Thiele H, Chatterjee S, Wollnik B, Nürnberg P. A systematic comparison of two new releases of exome sequencing products: the aim of use determines the choice of product. Biol Chem. 2016 Aug 1;397(8):791-801. doi: 10.1515/hsz-2015-0300.
  4. Kawalia A, Motameny S, Wonczak S, Thiele H, Nieroda L, Jabbari K, Borowski S, Sinha V, Gunia W, Lang U, Achter V, Nürnberg P. Leveraging the power of high performance computing for next generation sequencing data analysis: tricks and twists from a high throughput exome workflow. PLoS One. 2015 May 5;10(5):e0126321. doi: 10.1371/journal.pone.0126321. eCollection 2015.
  5. Motameny S, Wolters S, Nürnberg P, Schumacher B. Next Generation Sequencing of miRNAs - Strategies, Resources and Methods. Genes (Basel). 2010 Jun 3;1(1):70-84. doi: 10.3390/genes1010070.